Plasmids for therapy and vaccination



Publisher: Wiley-VCH in Weinheim, New York

Written in English
Cover of: Plasmids for therapy and vaccination |
Published: Pages: 287 Downloads: 159
Share This

Subjects:

  • Plasmids.,
  • DNA vaccines.,
  • Gene therapy.,
  • Plasmids.,
  • Vaccines, DNA.,
  • Gene Therapy.

Edition Notes

Includes bibliographical references and index.

Statementedited by M. Schleef.
ContributionsSchleef, M.
Classifications
LC ClassificationsQH452.6 .P566 2001
The Physical Object
Paginationxix, 287 p. :
Number of Pages287
ID Numbers
Open LibraryOL3972064M
ISBN 103527302697
LC Control Number2001281025
OCLC/WorldCa45487633

Downstream processing of plasmid DNA for gene therapy and DNA vaccine applications.   Introduction. The first reporting showing that the direct injection of naked plasmid DNA carrying eukaryotic genes into the mammalian muscle leads to endogenous expression of and to a specific immune response against the encoded protein were published some 25 years ago and provided the basis for the development of DNA-vaccines [1,2,3].These experiments DNA-based immunization Cited by:   The profiles we obtained for the two plasmids were fully conforming to the theoretical one. pDNA sequencing is also recommended [21] as a mean for quality control of plasmid preparations intended for clinical applications (i.e., gene therapy or DNA vaccination). Book Review Plasmids: Current Research and Future Trends Expert Rev. Vaccines 8(1), 33–34 () Editor: George Lipps Publisher: Caister Academic Press, Norfolk, UK ISBN: () Since their discovery in , plasmids have revolutionized science. Their initial impact on our understanding of micro-biology and bacterial.

Plasmids for Gene Therapy / Karthikeyan Kandavelou and Srinivasan Chandrasegaran --Ch. 8. Plasmid DNA as Prophylactic and Therapeutic Vaccines for Cancer and Infectious Diseases / Devin B. Lowe, Michael H. Shearer, Cynthia A. Jumper, En-Min Zhou and Ronald C. Kennedy.   DNA Vectors: From Research Tools to Pharmaceutical Substances. Since plasmids were identified initially as elements carrying extrachromosomal genetic information of Escherichia coli (E. coli) bacteria [], these typically circular DNA molecules became one of the most important tools in genetics and molecular biology and since the idea of gene therapy or genetic vaccination grew also Cited by: 4. Abstract The past several years have witnessed a rapidly increasing number of reports on utilizing plasmid DNA as a vector for the introduction of genes into mammalian cells for use in both gene therapy and vaccine applications. “Naked DNA vaccines” allow the foreign genes to be transiently expressed in transfected cells, mimicking intracellular pathogenic infection and triggering both the. Plasmid DNA processing for gene therapy and vaccination: Studies on the membrane sterilisation filtration step Journal of Membrane Science, Vol. , No. Direct capture of plasmid DNA from non-clarified bacterial lysate using polycation-grafted monolithsCited by:

Abstract. A thesis is presented which outlines investigations conducted to determine issues which may be faced should attempts be made to produce large plasmids (>kb) for use in areas such as DNA vaccination and gene therapy. DOI: /S(03) Corpus ID: Industrial scale production of plasmid DNA for vaccine and gene therapy: plasmid design, production, and purification @inproceedings{PratherIndustrialSP, title={Industrial scale production of plasmid DNA for vaccine and gene therapy: plasmid design, production, and purification}, author={Kristala Jones Prather and . A plasmid is a small, extrachromosomal DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently. They are most commonly found as small circular, double-stranded DNA molecules in bacteria; however, plasmids are sometimes present in archaea and eukaryotic nature, plasmids often carry genes that benefit the survival of the organism.

Plasmids for therapy and vaccination Download PDF EPUB FB2

The book describes modified vector systems based on plasmids, as well as the potency of genomic research and vector design by informatics.

Using the example of fish vaccination, the application of DNA vaccination in veterinary health care is reviewed, followed by a detailed overview of plasmid production technology on an industrial scale.

The book describes modified vector systems based on plasmids, as well as the potency of genomic research and vector design by informatics.

Using the example of fish vaccination, the application of DNA vaccination in veterinary health care is reviewed, followed by a detailed overview of plasmid production technology on an industrial : Hardcover.

Plasmids for Therapy and Vaccination. (Editor) ISBN: July Wiley-Blackwell Pages. E-Book $ O-Book. About the Author. Budding entrepreneurs, owners of bricks-and-mortar businesses who want to take their businesses online.

Table of contents. The Biology of Plasmids Structures of Plasmid DNA Genetic Vaccination Author: Martin Schleef. Shareable Link. Use the link below to share a full-text version of this article with your friends and colleagues.

Learn more. General properties of plasmids Plasmid‐encoded phenotypes The clinical importance of plasmids Plasmid cloning vectors The Biology of Plasmids - Plasmids for Therapy and Vaccination - Wiley Online LibraryCited by: Developments in gene therapy, cell therapy, and DNA vaccination require a pharmaceutical gene vector that, on one hand, fulfils the properties to express the encoded information-preferably at the.

Summary This chapter contains sections titled: Introduction Topological structures of plasmids Supercoiling of DNA DNA intercalating dyes Analysis of plasmid structures Conclusion Structures of Plasmid DNA - Plasmids for Therapy and Vaccination - Wiley Online LibraryCited by: Peter Mayrhofer, Martin Schleef and Wolfgang Jechlinger, Use of Minicircle Plasmids for Gene Therapy, Gene Therapy of Cancer, /_4, (), ().

Crossref Wolfgang Jechlinger, Optimization and delivery of plasmid DNA for vaccination, Expert Review of Vaccines, 5, 6, (), ().Cited by: 7. Plasmid DNA (pDNA) is the base for promising DNA vaccines and gene therapies against many infectious, acquired, and genetic diseases, including HIV-AIDS, Ebola, Malaria, and different types of cancer, enteric pathogens, and by:   Introduction.

Gene therapy approaches are of increasing interest, and >1, clinical trials have already been approved worldwide. 1 Since the discovery that plasmid DNA injection can be an efficient way to express a specific protein in vivo, 2 the so-called “nonviral” gene therapy field has been growing.

The production of plasmid DNA requires specific markers able to select plasmid Cited by: Plasmids for therapy and vaccination.

[M Schleef;] -- This is the first book specializing in plasmids and their biomedical use, including all relevant aspects of production, applications, quality, and regulations. Readers will discover clinical Your Web browser is not enabled for JavaScript. Plasmids for therapy and vaccination. [M Schleef;] -- This book summarizes the background of plasmids, presenting their structures and a set of clinical applications to provide an overview of the wide range of preventive and therapeutic applications.

The wide spread of infectious diseases have provoked the scientists to develop new types of vaccines. Among the different types of vaccines, the recently discovered plasmid DNA vaccines, have gained tremendous attentions in the last few decades as a modern approach of by: 4.

Book Review: Plasmids for Therapy and Vaccination. Edited by Martin Schleef Book Review: Plasmids for Therapy and Vaccination. Edited by Martin Schleef Kistner, Otfried BOOKS to construct modified vector systems for times less is better than more ; some Plasmids for Therapy and Vaccina- tion.

Edited by Martin Schleef. Plasmids for Therapy and Vaccination This is the first book specializing in plasmids and their biomedical use, including all relevant aspects of production, applications, quality, and regulations. Readers will discover clinical applications for the wide range of preventive and therapeutic applications using plasmid DNA.

Structures of Plasmid DNA. In book: Plasmids for Therapy and Vaccination, pp - 43 development and first clinical approaches to use DNA vectors in gene therapy, cell therapy and DNA.

Buy Plasmids for Therapy and Vaccination by Schleef, Martin (ISBN: ) from Amazon's Book Store. Everyday low prices and free delivery on eligible : Hardcover.

Plasmid DNA [] and now mRNA [] vaccines have generated significant interest and efforts because of their potential as platform technologies that could be used for a variety of applications ranging from prophylaxis to therapy and from personalized medicine to global health can be quickly made with fairly generic manufacturing processes and can be constructed directly from the Cited by: The use of plasmid DNA for vaccination and therapy is a relatively novel technology, with advantages and limitations as with other gene transfer techniques.

This is the first book specializing in plasmids and their biomedical use, including all relevant aspects of production, applications, quality, and regulations.

Readers will discover clinical applications for the wide range of preventive and therap. The demand for plasmid DNA has increased vastly in response to rapid advances in its use in gene therapy and vaccines. These therapies are based on the same principle, i.e.

the introduction of. Abstract: The demand for plasmid DNA has increased vastly in response to rapid advances in its use in gene therapy and vaccines.

These therapies are based on the same principle, i.e. the introduction of nucleic acids in human/non-human cells receptor to restore, cancel, enhance or Cited by: Downstream processing of plasmid DNA for gene therapy and DNA vaccine applications Guilherme N.M.

Ferreira, Gabriel A. Monteiro, Duarte M.F. Prazeres and Joaquim M.S. Cabral Interest in producing large quantities of supercoiled plasmid DNA has recently increased as a result of the rapid evolution of gene therapy and DNA vaccines.

Since then, intramuscular injection of plasmid DNA has been extensively used in gene therapy and in the design of novel DNA-based vaccines. To date, well over plasmid DNA-based gene therapy, cancer vaccine, and therapeutic vaccine clinical trials have been initiated.Cited by: Plasmid DNA pDNA gene therapy genetic vaccination purification manufacturing industrial-scale large-scale anion-exchange chromatography cGMP manufacturing QIAGEN pAlliance This is a preview of subscription content, log in to check by: Plasmid DNA has also been used to express specific antigens on cell membranes, stimulating and enhancing the immune system’s response and memory, and thereby offers the potential to generate a new, safer generation of vaccines 8.

Plasmid-DNA-based delivery vectors are, however, less effective at transfecting cells than the viral vectors by: Plasmids are covalently closed, double stranded, DNA molecules which carry genetic information. These macromolecules have been used for gene therapy and DNA vaccination, and belong to a new class of medicinal agents that contain genetic by: Interest in producing large quantities of supercoiled plasmid DNA has recently increased as a result of the rapid evolution of gene therapy and DNA vaccines.

Owing to the commercial interest in these approaches, the development of production and purification strategies for gene-therapy vectors has been performed in pharmaceutical companies within a confidential by:   Recent advances in chromatographic purification of plasmid DNA for gene therapy and DNA vaccines: A review.

Abdulrahman A(1), Ghanem A(2). Author information: (1)Chirality Program, Faculty of Science and Technology, University of Canberra, Cited by: 4. Transfection of cells, ear skin and detection of expressed proteins by Western blot and ELISA. HEK cells (8 × 10 5) were transiently transfected with 2 μg plasmid DNA by Fugene 6 Reagent (Roche, Indianapolis, IN) or by gene gun bombardment at psi (3 shots with 1 μg plasmid).After 24 h of growth (37 °C, 10% CO2) in Dulbecco’s Modified Eagles Medium supplemented Cited by:.

Plasmids are extra pieces of genetic material found in many cells that usually confer a specific property to the cell. These properties include antibiotic resistance, toxin production, and many other features. Plasmids are used in genetic engineering to generate recombinant DNAs and as a mechanism to transfer genes between organisms.

Minicircles are novel supercoiled minimal expression cassettes, derived from conventional plasmid DNA by site-specific recombination in vivo in Escherichia coli for the use in nonviral gene therapy and vaccination.

Minicircle DNA lacks the bacterial backbone sequence consisting of an antibiotic resistance gene, an origin of replication, and Cited by: Industrial scale production of plasmid DNA for vaccine and gene therapy: plasmid design, production, and purification Article in Enzyme and Microbial Technology 33(7) December with.